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Anglický jazyk
Amylase Production Through Agro-Waste
Autor: Shalini Rai
Increased production of a-amylase was observed with Bacillus amyloliquefaciens in the present study with wheat flour among the various agro-byproducts tested. The amylase from the bacterium was optimally active at a temperature of 37°C for 72h at pH 4±0.2... Viac o knihe
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O knihe
Increased production of a-amylase was observed with Bacillus amyloliquefaciens in the present study with wheat flour among the various agro-byproducts tested. The amylase from the bacterium was optimally active at a temperature of 37°C for 72h at pH 4±0.2 by using solid substrate (wheat flour) with the initial moisture of 80% and inoculated with Bacillus amyloliquefaciens of ¿2×106 cfu ml-1 of inoculum size. Supplementation with 0.04M KH2PO4, 1% maltose as a carbon source, tryptone and ammonium chloride as inorganic and organic nitrogen source in the substrate enhanced the enzyme yield considerably. The a-amylase from Bacillus amyloliquefaciens was partially purified by ammonium sulphate precipitation and ion exchange chromatography. Purification of amylase enzyme yielding a 2.05 fold purified enzyme with a specific activity of 11214.70 IU/mg. Characterization of purified enzyme revealed maximum stability on 65¿C temperature at pH 6±0.2. The enzyme was stable against Ca+2, Fe+3, Mn+2 metal ions however Cu+2 showed inhibitory effect. The characteristics of the enzyme qualify it as a good enzyme in biotechnological applications.
- Vydavateľstvo: LAP LAMBERT Academic Publishing
- Rok vydania: 2013
- Formát: Paperback
- Rozmer: 220 x 150 mm
- Jazyk: Anglický jazyk
- ISBN: 9783659487590
