Metabolic Flux Analysis in Eukaryotic Cells

Anglický jazyk

Metabolic Flux Analysis in Eukaryotic Cells

Vydavateľstvo: Springer US
Rok vydania: 2020
Formát: Hardback
Rozmer: 260 x 183 mm
Jazyk: Anglický jazyk
ISBN: 9781071601587

Na objednávku

145.19 €

bežná cena: 164.99 €

O knihe

This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols is a valuable resource for both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases.