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Anglický jazyk
Characterization of the CD83 promoter/enhancer complex
Autor: Marcello Stein
CD83 is one of the best-known surface markers for mature dendritic cells (DC). As CD83 is specifically upregulated upon DC maturation, a highly cell type- and maturation status-specific gene regulation is hypothesized. To elucidate the regulation mechanisms... Viac o knihe
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O knihe
CD83 is one of the best-known surface markers for mature dendritic cells (DC). As CD83 is specifically upregulated upon DC maturation, a highly cell type- and maturation status-specific gene regulation is hypothesized. To elucidate the regulation mechanisms of CD83 expression in human DC, ChIP-chip-microarray-, biocomputational-, reporter, CpG-methylation- and EMSA -analyses were performed. By these studies, a tripartite transcriptional activation complex composed of an upstream regulatory element, a minimal promoter and an enhancer was identified. Their specific arrangement and cooperation in trans, mediated by a complex framework of IRF- and NFkB-binding sites, has not been reported for any other gene so far. The characterization of the CD83 promoter complex not only allows a detailed look into the elaborate mechanisms of the immune response in general and the biology of DC in particular, but also exemplarily unfolds the cooperation of various promoter elements in a highly cell type- and status-specific immune cell related promoter. Thus, for the first time, a human mature DC-specific promoter is described that might become an important tool for transcriptional targeting of DC.
- Vydavateľstvo: Südwestdeutscher Verlag für Hochschulschriften AG Co. KG
- Rok vydania: 2015
- Formát: Paperback
- Rozmer: 220 x 150 mm
- Jazyk: Anglický jazyk
- ISBN: 9783838136684
