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Anglický jazyk
Plant beta galactosidases
Autor: Shajrul Amin
ß-galactosidases belong to glycosyl hydrolase family of enzymes.These enzymes are widely distributed in nature.ß-galactosidases have been implicated in many biological functions like hydrolysis of dietary lactose,degradation of glycolipids and proteoglycans,fruit... Viac o knihe
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O knihe
ß-galactosidases belong to glycosyl hydrolase family of enzymes.These enzymes are widely distributed in nature.ß-galactosidases have been implicated in many biological functions like hydrolysis of dietary lactose,degradation of glycolipids and proteoglycans,fruit ripening, cell elongation, storage mobilisation and petal senescence. ß - galactosidase has proven as a best alternative for elimination of lactose intolerance.The present study was aimed at investigating the biochemical properties of ß-galactosidases from apricots.Three isoenzymes(ß-gal I,ß-gal II and ß-gal III) were obtained by salt fractionation and ion-exchange and Sephadex G-100 column chromatography. ß-gal I,II and III showed 0.39, 0.52 and 0.30 units of activity respectively. The molecular weight of ß-gal I,II an III as determined by gel filtration was found to be 44.15,34.70 and 23.71 KDa respectively. The optimum pH for the activity of different isozymes was between 4 and 6. The isoenzymes were determined to be thermally stable upto 40oC. The Km for ß-gal I was 1.85mM,1.7mM for ß-gal II and 1.19 for ß-gal III.The Vmax for ß-gal I,ß-gal II aand ß-gal III was found to be 0.52,0.70 and 0.38µmole/min respectively.
- Vydavateľstvo: LAP LAMBERT Academic Publishing
- Rok vydania: 2011
- Formát: Paperback
- Rozmer: 220 x 150 mm
- Jazyk: Anglický jazyk
- ISBN: 9783846512333
