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Anglický jazyk
RNA Interference in insect cells infected with baculovirus
Autor: Tamer Salem
Baculovirus-insect cell system (BICS) is considered one of the most efficient eukaryotic gene expression systems. Establishing a universal gene silencing (UGS) system is very important due to the increasing number of studies using RNA interference (RNAi)... Viac o knihe
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O knihe
Baculovirus-insect cell system (BICS) is considered one of the most efficient eukaryotic gene expression systems. Establishing a universal gene silencing (UGS) system is very important due to the increasing number of studies using RNA interference (RNAi) with BICS. In this work, the enhanced green fluorescent protein (EGFP) was used as the RNAi consistent target sequence located downstream of a depressant insect-neurotoxin gene, LqqIT2 used as a model of the gene of interest. Small interfering RNA (siRNA) and inverted repeats of EGFP gene (IR-EG) were examined in targeting the EGFP-LqqIT2 (EL)-fusion mRNA or LqqIT2-EGFP (LE)-bicistronic mRNA for degradation. Suppression efficiencies using these inducers were examined transiently and stably in uninfected and infected insect Sf9 cells.
- Vydavateľstvo: LAP LAMBERT Academic Publishing
- Rok vydania: 2010
- Formát: Paperback
- Rozmer: 220 x 150 mm
- Jazyk: Anglický jazyk
- ISBN: 9783843351355
