RNAi and Small Regulatory RNAs in Stem Cells

Anglický jazyk

RNAi and Small Regulatory RNAs in Stem Cells

Vydavateľstvo: Springer New York
Rok vydania: 2017
Formát: Hardback
Rozmer: 260 x 183 mm
Jazyk: Anglický jazyk
ISBN: 9781493971060

Na objednávku, dodanie 2-4 týždne

158.39 €

bežná cena: 175.99 €

O knihe

This detailed collection provides an accessible compendium of up-to-date methods focused on the study of RNAi and small regulatory miRNAs in stem cells. Beginning with a brief introductory section, the volume continues by exploring methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, recently developed methods for miRNA expression and functional analysis, as well as usage of CRISPR/Cas 9 to knockout an individual gene for functional studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, RNAi and Small Regulatory RNAs in Stem Cells: Methods and Protocols aims to accelerate progress in this crucial field by reducing the time required to decipher and put into practice procedures published in the literature.